Stealth coating of NPs with zwitterionic lipid bilayers

The immune response triggered by the nanoparticles limits their activity and effectiveness when injected into the body. This reaction is mainly caused by the adsorption of proteins in the nanoparticles (NPs) which depends on the physicochemical characteristics of the NPs surface and varies between different materials. To control surface fouling and the immune response, zwitterionic-rich lipid bilayer coatings will be used.

These lipid bilayers offer advantageous physicochemical properties

Firstly, they are the main molecules of the cell membranes thus they are highly biocompatible. Secondly, they have anti-fouling properties given by the hydration layer which reduces nonspecific protein adsorption. Moreover, the composition of the bilayer can be easily tuned with the addition of different lipids to modulate surface charge and even add functionalized lipids for specific targeting. 

During the project, different NPs will be coated and an extended study of the immune system response will be performed with the help of the other early-stage researchers. The different studies will include amongst others whole blood assays to study T-cell and macrophage (subtype) activation. We anticipate that a successful coating will homogenize the distinct immune response to the respective NPs.


Mireia Vilar Hernandez - Early Stage Researcher

LipoCoat BV (The Netherlands)


Perspectives on complement and phagocytic cell responses to nanoparticles: From fundamentals to adverse reactions

The complement system, professional phagocytes and other cells such as Natural killer cells and mast…
read more

Nanometer- and angstrom-scale characteristics that modulate complement responses to nanoparticles

The contribution of the complement system to non-specific host defence and maintenance of homeostasis is…
read more

A brief history of long circulating nanoparticles

Kupffer cells rapidly intercept colloidal particles from the blood. Early studies show predosing with placebo…
read more
view all topics
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 956544
© 2024 University of Padova - All rights reserved P.Iva 00742430283